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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
Phalaenopsis Aphrodite Subspecies Formosana, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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Taiwan Sugar Corporation p. aphrodite subsp. formosana plants
The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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Technik GmbH veil of aphrodite–technik
The donor plants of P. aphrodite subsp. <t>formosana</t> , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).
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The donor plants of P. aphrodite subsp. formosana , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).

Journal: The Scientific World Journal

Article Title: A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants

doi: 10.1155/2014/263642

Figure Lengend Snippet: The donor plants of P. aphrodite subsp. formosana , their seed germination, and direct somatic embryogenesis from intact seedlings. (a) A flowering potted plant for self-pollination to obtain seeds ( bar 1.5 cm). (b) In vitro seed germination after 1 month of sowing on 1/4 MS medium ( bar 1 mm). (c) A cluster of embryos originated from the posterior region of a 2-month-old seedling ( bar 3 mm). (d) The embryos turned into protocorm-like bodies ( bar 2.5 mm). (e) The early event of embryogenesis originated from the leaf surface of a 2-month-old seedling ( bar 3.5 mm). (f) The foliar embryos formed from an intact 2-month-old seedling ( bar 2 mm). (g) The foliar embryos developed leaves while they were still connected with the first leaf of the parent seedling ( bar 5 mm). (h) Numerous embryos initiated from almost the entire seedling (bar = 4 mm). (i) Plantlet conversion while the embryos were still on the parent seedling ( bar 4.5 mm). (j) Two-month-old rooted plantlets were successfully established ( bar 1 cm).

Article Snippet: To our best knowledge, the species used this present study; Phalaenopsis aphrodite subspecies formosana (formerly P. amabilis variety formosa Shimadzu) is qualified to be a model plant in the recent orchid research which has been intensively studied in the past 10 years, including in vitro protocols, flowering and photosynthetic physiology, chloroplast genomic analysis, global analysis of transcriptome, and modified ABCDE model of flowering [ , – ].

Techniques: In Vitro